![]() ![]() Quality Control :ĥ μl of BlueRAY Prestained Protein Ladder (PM006-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane. Contents :Īpproximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25☌), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) glycerol. Apply more for thicker (>1.5 mm) or larger gel.2~3 μl per well for general Western transferring.3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.Migration patterns of BlueRAY Prestained Protein Ladder in different electrophoresis conditions are listed below : Guide for Molecular Weight Estimation (kDa) Approximately 0.10.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH. They are detected by incubating the blot with Europium-labeled streptavidin and they show as light colored bands when read with the ScanLater Western Blot Detection Cartridge. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading. The ScanLater Western Blot Protein Ladder consists of seven biotinylated recombinant proteins at 10, 20, 40, 50, 80, 100 and 140 kD. Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / 9 bands / SDS-polyacrylamide gel electrophoresis Categories: Protein Marker/Ladder. ![]() ![]() The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated by SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. ![]()
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